DEPOLARIZATION OF END-PLATES BY ACETYLCHOLINE EXTERNALLY APPLIED

Control of sawtooth oscillation dynamics using externally applied stellarator transform

The control of sawtooth oscillations is an active area of tokamak research. Large sawtooth oscillations need to be avoided in ITER, since these large sawteeth couple to neoclassical tearing modes and edge localized modes resulting in serious confinement degradation. Small sawtooth oscillations, however, may be beneficial in preventing impurity and helium ash accumulation in the center of the pl...

Inhibition by botulinum toxin of depolarization-evoked release of (14C)acetylcholine from synaptosomes in vitro.

1. Cerebral-cortex synaptosomes were shown to synthesize (14C)acetylcholine after incubation with (14C)choline, and 25mM-KCl released (14C)acetylcholine (but not (14C)choline) into the medium by a Ca2+-dependent and Mg2+-sensitive process. 2. The K+-stimulated release of (14C)acetylcholine was inhibited by more than 80% after preincubation of the synaptosomes with 10(5) mouse lethal doses of bo...

Control of microtubule assembly by extracellular matrix and externally applied strain.

A number of studies have suggested that externally applied mechanical forces and alterations in the intrinsic cell-extracellular matrix (ECM) force balance equivalently induce changes in cell phenotype. However, this possibility has never been directly tested. To test this hypothesis, we directly investigated the response of the microtubule (MT) cytoskeleton in smooth muscle cells to both mecha...

Regulation of muscarinic acetylcholine receptor number in cultured neuronal cells by chronic membrane depolarization.

The effect of chronic membrane depolarization on the regulation of muscarinic acetylcholine receptor (mAChR) number was studied in neuroblastoma cells (clone N1E-115). Receptor number was determined by a filter binding assay using 3H-quinuclidinyl benzilate (QNB) in membrane and crude cellular homogenates. Incubation with 50 microM veratridine (VTN), an activator of voltage-sensitive Na+ channe...

Low temperature crystallization of germanium on plastic by externally applied compressive stress